安徽农业科学2012，Vol.40Issue(15)：8738-8739,8781,3.
荔枝壳总黄酮大孔树脂纯化工艺研究
Separation and Purification Process of Total Flavones from Litchi Pericarp by Macroporous Absorption Resin
摘要
Abstract
[ Objective ] This study aimed to optimize the conditions for purification of total flavones from litchi pericarp by macroporous absorption resin. [Method] The flavones adsorption rate and desorption rate of macroporous absorption resins (AB-8, HPD-600, D101) were compared,and the technological parameters of D101 during the purification process were investigated,including on-column liquid pH,flavone-concentration,on-column liquid volume and volume fraction of elution solution. [Result] D101 macroporous absorption resin is appropriate for the purification of total flavonoids from litchi pericarp. The optimal technological conditions were;on-column liquid pH =5.0; on-column liquid concentration was 4 mg/ml,with a volume of 2.5 BV; elution solution was 80% ethanol,with a volume of 2.0 BV. [Conclusion] The content of total flavones achieve 83% by using the method.关键词
荔枝壳/总黄酮/大孔吸附树脂/纯化工艺Key words
Litchi pericarp/ Total flavones/ Macroporous absorption resin/Purification process分类
农业科技引用本文复制引用
涂华,陈碧琼,周锡兰..荔枝壳总黄酮大孔树脂纯化工艺研究[J].安徽农业科学,2012,40(15):8738-8739,8781,3.基金项目
泸州市重点科技项目(651). （651）
