安徽农业科学2012，Vol.40Issue(16)：8811-8813,3.
Sus scrofa interferon epsilon-1基因的克隆与序列分析
Cloning and Sequence Analysis of Sus scrofa interferon epsilon-1 Gene
杜会坡 1台玉磊 2王伟杰 3杨国宇2
作者信息
- 1. 伊川县动物疫病预防控制中心,河南洛阳471300
- 2. 河南农业大学动物生理生化实验室,河南郑州450002
- 3. 洛阳市动物疫病预防控制中心,河南洛阳471002
- 折叠
摘要
Abstract
[ Objective] To clone and analyze the Sus scrofa interfemn epsiton-I gene, so as to lay foundation for the study of its biological func-tions. [ Method] The swine HTG database was searched with the Homo sapiens interferon epsilon-I (1FNEI) sequence (NM_176891.3) , two fragments (CU074336 and AC127471) were obtained and analyzed by sequencing. One pair of primers was cloned at 5'-UTR and 3'-UTR to analyze the gastric tissue of seven-day-old piglets by RT-PCR, the PCR products were cloned, sequenced and correlation analyzed. [ Result] The homology analysts showed that the homology of swine SIFNEI with human and mice interferon epsilon-I gene cDNA CDS was 83.6% and 69.2% respectively, the homology between their protein sequences was 76. 2% and 55.2% respectively. The signal peptide of ami no acid se-quence was predicted to be the 1 - 12 position of amino acid, the protein domains of IFabd were the 59 - 176 position of amino acid, which was consistent with the interferon epsilon-I of human and mice. [Conclusion] The study cloned the Sus scrofa interferon epsilon-I gene, and laid foundation for furthur studying its biological functions.关键词
猪/SIFNEI/克隆/序列分析Key words
Swine/ SIFNE1/ Cloning/ Sequence analysis分类
农业科技引用本文复制引用
杜会坡,台玉磊,王伟杰,杨国宇..Sus scrofa interferon epsilon-1基因的克隆与序列分析[J].安徽农业科学,2012,40(16):8811-8813,3.
