安徽农业科学2012，Vol.40Issue(16)：8830-8832,3.
改良热酚法快速提取棉花不同组织RNA
Rapid Extraction of Total RNA from Different Cotton Tissues by Modified Hot-phenol Method
摘要
Abstract
[ Objective] This study aimed to develop a hot-phenol method for extracting the total RNA from different cotton tissues. [ Method] The total RNA of different tissues of Cossypium hirsutum cultivar Xinluzao 26 was extracted with cold-phenol method, Trizol method, modified hot-phenol method and CTAB method, respectively; all the extracted RNA samples were analyzed with electrophoresis and compared. [Re-sult] The RNA samples extracted by modified hot-phenol method were complete, high-concentration, free from contaminations by polysaccha-rides and polyphenols, and could be used for rapid-amplification of cONA ends, northern blot, gene chip analysis and Iranscriplome analysis, etc. [Conclusion] This method can be adopted lo extract high-quality total RNA from cotton leaves, root, shoot, corolla, anther, ovule and developing fibers. Meanwhile, it is simple, low-cost and produces Little pollution to environment.关键词
RNA/棉花/多糖多酚/纯度Key words
RNA/ Cotton/ Polysaccharides and polyphenols/ Purity分类
农业科技引用本文复制引用
司爱君,阮孟斌,谢宗铭..改良热酚法快速提取棉花不同组织RNA[J].安徽农业科学,2012,40(16):8830-8832,3.基金项目
新疆兵团博士基金(2008JC03) （2008JC03）
转基因生物新品种培育重大专项(2009ZX08009-090B) （2009ZX08009-090B）
兵团青年科技创新资金(2011CB001). （2011CB001）
