安徽农业科学2012，Vol.40Issue(16)：8915-8917,3.
犬瘟热病毒的分离与鉴定
Isolation and Identification of Canine Distemper Virus
刘芳园 1乔军 1张银国 1倪伟 1孟仁 1于振兴1
作者信息
- 1. 石河子大学动物科技学院,新疆石河子832003
- 折叠
摘要
Abstract
[ Objective ] To isolate and identify the epidemic strains of Canine Distemper Virus ( CDV) in Shihezi. [ Method ] The lymph node9 of the nature morbidity dog with suspected clinical symptoms of canine distemper and positive results for ELISA assay were sampled tobe inoculated into the African green monkey kidney cell line (Vero) to isolate canine distemper virus. RT-PCR was used to delect CDV virus nucleic acid to identify the infected virus in cells. [ Result] The pathological material vaccinated into the Vero cell produced obvious cell pathological change (CPE) , with the RT-PCR technology, the CDV specific nucleic acid was amplified, whose length was the same as the predicted, the nueleotide homology between the H gene fragment of CDV isolates and the C54 standard virulent strains of CDV was 98.4%. [ Conclusion] The CDV was successfully isolated and identified to be an epidemic strains of Shihezi, providing references for the diagnosis of CD.关键词
犬瘟热病毒/分离/鉴定Key words
Canine distemper virus / Isolation/ Identification分类
农业科技引用本文复制引用
刘芳园,乔军,张银国,倪伟,孟仁,于振兴..犬瘟热病毒的分离与鉴定[J].安徽农业科学,2012,40(16):8915-8917,3.
