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西瓜不同生长阶段的过氧化氢酶活性研究

杨冬业 张丽珍 徐淑庆

安徽农业科学Issue(18):9604-9606,3.
安徽农业科学Issue(18):9604-9606,3.

西瓜不同生长阶段的过氧化氢酶活性研究

Study on Catalase Activity in Watermelon during Its Different Growth Periods

杨冬业 1张丽珍 2徐淑庆3

作者信息

  • 1. 桂林医学院,广西桂林541004
  • 2. 桂林师范高等专科学校,广西桂林541001
  • 3. 钦州学院生化系,广西钦州535000
  • 折叠

摘要

Abstract

[Objective] To determine the catalase activity in watermelon during its different growth periods, so as to lay experimental basis for studying its metabolic changes and growing vitality at different periods. [ Method] Catalase was extracted from watermelon seeds during its ger-minating period, seedling period, seedling formation period, cotyledon differentiating period and adventitious bud growing period, and then the eatalase activity was detemtined with UV spectrophotometer. [ Result] The catalase activity in watermelon was the highest during the germina-ting period, while the lowest during the seedling formation period, the catalaac activity at different growth periods decreased in the order of ger-minating period > cotyledon differentiating period > seedling period > adventitious bud growing period > seedling formation period. The cat-alase activity in watermelon seedlings was gradually decreasing during its normal growth period, and it was also decreasing during the growth period of adventitious buds. [ Conclusion] The experiment supplied a method for determining the eatalase activity in plants, and also laid foun-dation for studying the antioxidant capacity of organisms under natural environment.

关键词

西瓜/组织培养/过氧化氢酶/分光光度法

Key words

Watermelon/Tissue culture/Catalase/Spectrophotometry

分类

农业科技

引用本文复制引用

杨冬业,张丽珍,徐淑庆..西瓜不同生长阶段的过氧化氢酶活性研究[J].安徽农业科学,2012,(18):9604-9606,3.

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