安徽农业科学Issue(18)：9639-9640,9668,3.
金槐米槲皮素对人鼻咽癌细胞的干预作用研究
Intervention Effects of Quercetin from Sophora japonica on the Human Nasopharyngeal Carcinoma Cell
摘要
Abstract
[Objective] To discuss the intervention effects of Sophora japonica quercetin on the human nasopharyngeal carcinoma cell CNE2. [ Method] Quercetin was extracted from S. japonica and then purified by microwave-assisted extraction. Quercetin was used to interfere human nasopharyngeal carcinoma cell CNE2 with the concentrations of 20, 40, 60 and 80 祄ol/L. After 24, 48 and 72 h, the inhibitory situation of human nasopharyngeal carcinoma cell CNE2 was preliminarily detected by MTT method. Cell cycle and apoptosis rate were analyzed by flow cytometry; and morphological changes were observed by optical microscope. [Conclusion] Quercetin extracted from S. japonica had significantly inhibitory effects on the human nasopharyngeal carcinoma cell, and showed obvious concentration and time dependent manners. The optimal concentration of drug intervention was 60 ujnol/L. After drug intervention, the optimal morphological observation time was 48 h. Cell cycle analysis showed that quercetin was able to block nasopharyngeal carcinoma cell at Gl period. [ Conclusion] Quercetin was extracted from S. japonica had intervention effects on human nasopharyngeal carcinoma cell.关键词
金槐米/槲皮素/人鼻咽癌Key words
Sopkora japonica/Quercetin/Nasopharyngeal carcinoma cells分类
农业科技引用本文复制引用
顾生玖,刘建楠,姚丽新,杨娜,朱开梅..金槐米槲皮素对人鼻咽癌细胞的干预作用研究[J].安徽农业科学,2012,(18):9639-9640,9668,3.基金项目
广西科技攻关项目(科技攻关0815005-1-17 （）
0992003A-25 （）
09321056)资助 （）
桂林市科技攻关项目(科技攻关20110106-5). （科技攻关20110106-5）
