安徽农业科学Issue(18)：9648-9650,3.
天麻多糖的提取工艺及含量测定研究
Extraction Technology of Polysaccharides from RHIZOMA GASTRODIAE and Its Content Determination
摘要
Abstract
[Objective] To establish n convenient and accurate method for the content determination of polysaccharides from RHIZOMA GAS- TRODIAE. [Method] Eatxaction process of RHIZOMA GASTRODIAE polysaccharides was optimized by orthogonal test. The total asccharide content, uronic acid content and protein content were detected by phenol.sulfuric acid method, m-hydroxydiphenyl method, and Comaasie Brilliant method, respectively. [Resuh] The optimal extraction condition was A2B2C3, that is 70 ℃ extraction temperature, 2.5 h extraction time, and 3 extraction times. Under this condition, the extraction rate of polysaccharides reached 13.15%. Saccharide contents in different groups of RHIZOMA GASTRODIAE polysaccharides we,re 65.8%, 84.6% and 87.3% ; protein contents were 2.9%, 0. 28% and 2.7% ; umnic acid contents were 29.7%, 26. 3% and 39. 6%. [Conclusion] This method was rapid, simple, accurate, and sensitive, and could be used for the quality control of RHIZOMA GASTRODIAE polysaccharidas.关键词
天麻/多糖/分光光度法/含量测定Key words
RHIZOMA GASTRODIAE/Polysacchorides/Content determination/Spectrophotometry分类
农业科技引用本文复制引用
朱洁平,李峰,沈业寿..天麻多糖的提取工艺及含量测定研究[J].安徽农业科学,2012,(18):9648-9650,3.基金项目
安徽省高等学校省级自然科学研究项目(KJ2012B209). （KJ2012B209）
