安徽农业科学Issue(18)：9655-9656,2.
正交设计优选喜树碱及10-羟基喜树碱的提取工艺
Optimization of Extraction Process of Camptothecin and 10-hydrxycamptothecin by Orthogonal Design
摘要
Abstract
[Objective] To select the ultrasonic optimal extraction technology of carnptotbeein( CPT)and IO-hydrxyeamptothecin( HCPT)from Camptotheca acuminate Decne fruits. [Method] Contents of camptothecin and 10-hydrxyeamptothecin were detected by high efficiency liquid ehromatography. Based on single factor test, L9 (34) orthngooal test was conducted to determine the optimal extraction condition. [ Resuh] The optimal extraction condition of camptothecin was as follows: 70% methanol, 1:30(g/ml) solid-liquid ratio, 60 ℃ extraction temperature, and 25 rain extraction time. The optimal extraction condition of 10-hydrxycamptothecin was as follows: 60% methanol, 1: 30(8/nd) solid-liquid ratio, 60 ℃ extraction temperature, and 20 rain extraction time. Verification test showed that the average extraction rates of camptothecin and 10-hydrxycamptothecin were 0.719‰ and 0. 238‰, with RSD being 1.99% and 1.79%, respectively. [ Conclusion] This method was stable and feasible. And the optimal extraction technologies of camptothecin and 10-hydrxycarnptothecin born C. acuminate frutits were obtained.关键词
喜树(Camptotheca acuminata)/喜树碱/10-羟基喜树碱/提取工艺/正交试验Key words
Camptotheca acuminata Decne/Camptothecing/10-hydroxycamptothecine/Extraction process/Orthogonal test分类
农业科技引用本文复制引用
赵金良,唐晓丹,运达,杭海洋..正交设计优选喜树碱及10-羟基喜树碱的提取工艺[J].安徽农业科学,2012,(18):9655-9656,2.基金项目
辽宁省自然科学基金项目(201102091) （201102091）
辽宁科技大学示范性开放实验室项目(2011006) （2011006）
辽宁科技大学大学生科研训练项目(第七期). （第七期）
