安徽医科大学学报2012，Vol.47Issue(9)：1011-1014,4.
抗Sj14-3-3特异性IgY的制备及其诊断日本血吸虫病循环抗原的价值
Preparation of specific yolk immunoglobulin (IgY) of Anti-Sj14-3-3 in serological diagnosis of Schistosomiasis japonica
摘要
Abstract
Objective To detect the diagnostic value of using yolk immunoglobulin ( IgY ) and establishing sandwich ELISA in Schistosoma japonicum. Methods IgY and IgG antibodies were prepared and purified from the yolk and the sera of rabbits immunized with rSj4-3-3. Double antibody sandwich ELISA using the specific IgG for capture and IgY for detection was set up to test sera of 72 cases of acute and 128 cases of chronic schistosomiasis. 132 sera of normal individuals were used as control. Additionally, 42 patients with paragonimiasis, 60 with clonorchiasis and 46 with hookworm infections were subjected to cross-reaction investigation. Results The positive rates of acute and chronics schistosomiasis were 73. 6% and 48. 4% when IgY was used as detection antibody. The negative rate of normal human sera was 94. 7% ; the cross-reactions with paragonimiasis , clonorchiasis, and hookworm infections were found to be 7. 1% , 6. 7% , and 6. 5% , respectively. Conclusion IgY based double antibody sandwich ELISA used for the detection of circulating antigens has a potential and practical value in the diagnosis of schistosomiasis.关键词
日本血吸虫病/循环抗原/14-3-3蛋白/卵黄抗体Key words
Schistosomiasis japonicum/circulating antigen/signaling protein 14-3-3/yolk immunoglobulin分类
医药卫生引用本文复制引用
罗庆礼,周银娣,沈继龙..抗Sj14-3-3特异性IgY的制备及其诊断日本血吸虫病循环抗原的价值[J].安徽医科大学学报,2012,47(9):1011-1014,4.基金项目
国家高技术研究发展计划资助项目(编号:2006AA02A251) （编号:2006AA02A251）
安徽高校省级自然科学研究计划项目(编号:KJ2008B285) （编号:KJ2008B285）
