安徽医科大学学报2012，Vol.47Issue(9)：1024-1027,4.
IL-15对HaCaT细胞增殖的影响及机制研究
Study on the impact of IL-15 on proliferation of HaCaT cells
吴德全 1罗欣 2贾波 3程丰伟 3张胜权 3邓松华3
作者信息
- 1. 安徽医科大学病理生理学教研室,合肥,230032
- 2. 安徽医科大学第二附属医院护理部,合肥,230601
- 3. 安徽医科大学生物化学教研室,合肥,230032
- 折叠
摘要
Abstract
Objective To explore the impact of IL-15 on proliferation of HaCaT cells. Methods HaCaT cells were cultured and treated with various concentration of IL-15 for different time. MTT method was used to analyze the proliferation of HaCaT cells. Furthernore, we analyzed signaling pathways induced by IL-15 in HaCaT cells by Western-Blot , and then used the specific inhibitors to selectively inhibit ERK and AKT phosphorylation to reveal the relationship between the affect of IL-15 on the proliferation and these signaling pathways. Results In HaCaT cells, IL-15 promotes the proliferation of HaCaT cells at a dose-dependent and time-dependent ways. Mechanistic studies show that IL-15 increased the phosphorylation of ERK1/2 and AKT, and the specific inhibitors of the phosphorylation of ERKl/2( PD98059 ) or AKT ( LY2940002 ) could antagonize the effect of IL-15 on the proliferation of HaCaT cells. Conclusion The IL-15 partially depends on the MAPKs-ERKl/2 and PI3K-AKT signaling pathways to regulate the proliferation in HaCaT cells. 关键词
HaCaT细胞/IL-15/MAPKs-ERK1/2/PI3K-AKT/细胞增殖Key words
HaCaT cells/IL-15/MAPKs-ERKl/2/PI3K-AKT/cell proliferation分类
医药卫生引用本文复制引用
吴德全,罗欣,贾波,程丰伟,张胜权,邓松华..IL-15对HaCaT细胞增殖的影响及机制研究[J].安徽医科大学学报,2012,47(9):1024-1027,4.
