安徽医科大学学报2012，Vol.47Issue(9)：1124-1127,4.
优化组织块法体外培养新生SD大鼠原代成骨细胞与鉴定
New SD rat osteoblasts by modified explant culture in vitro and identification
摘要
Abstract
The original generation of osteoblasts of 24-hour old SD rats were isolated and cultured in vitro with three methods-the traditional tissue plant method, traditional enzyme digestion method, and modified enzymatic digestion method. The osteoblasts generated by 3 methods were identified and compared with each other at last. The modified enzymatic digestion method can train a lot of high purity osteoblasts in a short term, which cells had typical morphological and biological characteristics of osteoblasts. The ALP dyes and type I collagen immunity fluorescence were all positive. The activity of ALP reached 92%. This method is better than the other two, and provides a feasible, simple operation of the generation osteoblast training method in vitro.关键词
成骨细胞/原代培养/鉴定Key words
osteoblast/primary culture/identification分类
医药卫生引用本文复制引用
陈强,夏天,叶哲伟,宋谋珂..优化组织块法体外培养新生SD大鼠原代成骨细胞与鉴定[J].安徽医科大学学报,2012,47(9):1124-1127,4.基金项目
湖北省自然科学基金(编号:0202040401) （编号:0202040401）
