北华大学学报：自然科学版2012，Vol.13Issue(3)：296-300,5.
复发性外阴阴道假丝酵母菌病菌种分子分类与药敏的研究
Analysis on Molecular Taxonomy and Drug Sensitivity of Saccharomycete Species Causing Recurrent Vulvovaginal Candidiasis
摘要
Abstract
Objective To investigate the classification method of saccharomycetes collected in clinic and the feature of sensitivity to drugs. Method 93 strains of saccharomycetes causing recurrent vulvovaginal candidiasis were collected in clinic, France merry biological medicine sensitive kit, ATBTM FUNGUS3 ,was used for the drug sensitivity test, and the molecular classification method for the saccharomycete was studied based on ribosome gene 26S rDNA D1 / D2 sequence analysis. Results 93 strains of saccharomycetes were classified as Candida glabrata （ 38/93 ）, Candida albicans （ 23/93 ）, Candida parapsilosis （ 16/93 ）, Candida glabrata-like saccharomycete （9/93） and they included nine species and 11 gene types. 18 Strains of them were sensitive and 3 strains were completely resistant to the drugs. The sensitivity test for 5 frequently used antimycotic drugs showed that 5 FC was 83.87% , AMB was 93.55% , FCA was 27.96% , ITR was 21.51% and VRC was 43.01%. Conclusion Based on the molecular taxonomy of ribosome gene, it is possible accurately to identify the saccharomycetes collected in clinic. The feature of saccharomycetes causing recurrent vulvovaginal eandidiasis should be primarily Candida glabrata and Candida albicans accounts for 75.27%. The sensitivity of 93 strains of saccharomycetes to AMB and 5 FC should be higher than that of them to the triazole drugs. Among the triazole drugs, the resistant rate of saccharomycetes to VRC should be higher than that of them to FCA and ITR. It is deduced that drug sensitivity could be associated with strains.关键词
酵母菌/26S/rDNA/D1/D2/敏感性/耐药Key words
Saccharomycete/26 S rDNA D1/D2/sensitivity/resistance分类
医药卫生引用本文复制引用
迟绍琴,庄严,黄兴国,王玥红,陈奕微,吴志丽..复发性外阴阴道假丝酵母菌病菌种分子分类与药敏的研究[J].北华大学学报：自然科学版,2012,13(3):296-300,5.基金项目
广东省人口与计生委项目 （）
深圳市龙岗区科技计划医疗卫生项目 （）
