北京中医药大学学报2012，Vol.35Issue(2)：109-111,116,封3,5.
黄芪注射液对肾小管上皮细胞转分化的影响
Influence of Huangqi Injection on tubular epithelial-myofibroblast transdifferentiation
摘要
Abstract
Objective To observe the influence of Huangqi Injection on the epithelial-myofibroblast transdifferentiation (EMT) induced by transforming growth factor pi ( TGF-|31) in human renal tubular epithelial cells (HK-2) cultured in vitro, and reveal the detail effective mechanism in the treatment of renal interstitial fibrosis (RIF). Methods HK-2 cells were divided into 3 groups, and in normal group HK-2 cells were normally cultured, in model group, stimulated with TGF-pi (8 x 10 -9 mg/L) for 48 hours, and in treatment group, cultured together with TGF-pl (8 x 10 -9 mg/L) and Huangqi Injection (8 x 10 -6 mg/L) for 48 hours. The morphologic changes of HK-2 were observed with the inverted phase contrast microscope, and expressions of E-cadherin and a-smooth muscle actin ( a-SMA) were observed by using immunohistoehemistry assay. Results The observation results of the inverted phase contrast microscope showed that the morphologic changes experienced from epithelial-like transform to fibroblast-like transform in model group, and fibroblasts decreased and a lot of epithelioid cells were observed in treatment group. The results of immunohistochemistry assay showed that the expression of a-SMA increased and expression of E-cadherin decreased in model group compared with normal group, while in treatment group, expression of E-cadherin increased and expression of a-SMA decreased compared with model group. Conclusion Huangqi Injection can inhibit to a certain extent the phenotypic alternation induced by TGF-pl in HK-2 cells, reduce the expression of a-SMA, improve the expression of E-cadherin, and block or reverse EMT progress.关键词
人肾小管上皮细胞/上皮细胞转分化/黄芪注射液Key words
human renal tubular epithelial cells/ epithelial-myofibroblast transdifferentiation/ Huangqi Injection分类
医药卫生引用本文复制引用
李玉杰,王谦,来媛媛,李姝玉,吴莹,贾德贤,崔蕾,杨辉,柴新楼,李丽娜..黄芪注射液对肾小管上皮细胞转分化的影响[J].北京中医药大学学报,2012,35(2):109-111,116,封3,5.基金项目
国家自然科学基金资助项目(No.30672756,81072926),北京中医药大学创新团队资助项目(No.2011-CXD-04),北京中医药大学自主选题资助项目(No.2009JYBZZ-XS021) （No.30672756,81072926）
