北京中医药大学学报2012，Vol.35Issue(3)：169-173,5.
STAT3在附子多糖后处理保护缺氧/复氧乳鼠心肌细胞机制中的作用
Effect of STAT3 in mechanism of fuzi polysaccharides postconditioning protecting cardiomyocytes with hypoxia-reoxygenation in neonate rats
摘要
Abstract
Objective To investigate the protective mechanism of fuzi polysaccharides ( FPS ) postconditioning to cardiomyocytes with hypoxia-reoxygenation ( H/R) taking signal transducers and activators of transcription 3 (STAT3) as entry point. Methods The model of cardiomyocytes with H/R was established in neonate rats and then divided into normal group, H/R group, FPS group, Stattic (specific blocker of STAT3 ) and FPS group (Stattic + FPS group) and Stattic group. The apoptosis rate of myocardiocytes was detected by using flow cytometer, expressions of phosphorylation STAT3 (P-STAT3), cytochrome C(CytC)and caspase-3 were detected by applying Western blotting and expressions of Bcl-xl mRNA and Bcl-xs mRNA were detected by using fluosescence quantitative PCR. Results FPS postconditioning improved P-STAT3 expression, up-regulated Bcl-xl mRNA expression and down-regulated Bcl-xs mRNA expression, blocked the release of CytC and caspase-3 expression, and inhibited myocardiocyte apoptosis. Stattic can reverse the protective effect of FPS to cardiomyocytes and increase of P-STAT3 expression. Conclusion The protective effect of FPS to cardiomyocytes with H/R is related to the activation of STAT3, increase of Bcl-xl expression, protection of mitochondria, and blockage of mitochondria pathway of apoptosis.关键词
附子多糖/信号转导子与转录激活子3/缺氧/复氧损伤/后处理/乳鼠心肌细胞Key words
fuzi polysaccharides/STAT3/hypoxia-reoxygenation injury/postconditioning/cardiomyocytes of neonate rats分类
医药卫生引用本文复制引用
刘颖,纪超,吴伟康..STAT3在附子多糖后处理保护缺氧/复氧乳鼠心肌细胞机制中的作用[J].北京中医药大学学报,2012,35(3):169-173,5.基金项目
广东省科技计划资助项目(No.2008B060600055) （No.2008B060600055）
