北京中医药大学学报2012，Vol.35Issue(3)：174-177,4.
益气养阴消癥通络中药对高糖联合AngⅡ培养大鼠肾小球系膜细胞分泌转化生长因子β1和结缔组织生长因子的影响
Influences of Chinese medicinal on TGF-β1 and CTGF secreted by mesangial cells in rats fostered by high glucose combining Ang Ⅱ
摘要
Abstract
Objective To investigate the influences of Chinese medicinal with actions of replenishing qi and nourishing yin, and resolving mass and freeing collateral vessels on transforming growth factor p, (TGF-p,) and connective tissue growth factor (CTGF) secreted by mesangial cells (MC) in rats fostered by high glucose combining Ang II, and provide a foundation for further study on prevention and treatment of diabetic nephropathy (DN). Methods The fifth generation of primary cultured MC was stimulated with high glucose and high glucose combing Ang II as well as Chinese medicinal serum. After 48 hours, the levels of TGF-p,, fibronectin (FN) and laminin (LN) were detected by using ELISA, and expression of CTGF was detected by using Western blotting method. Results Compared with control group, the levels of TGF-β1 ,FN and LN and CTGF expression increased significantly in other groups. Compared with high glucose + Angll group, the levels of TGF-β1, FN and LN and CTGF expression decreased significantly in irbesartan group, Chinese medicinal serum group (P < 0. 01). Conclusion Chinese medicinal with actions of replenishing qi and nourishing yin, and resolving mass and freeing collateral vessels can effectively decrease the level of TGF-β1, inhibit the composition of extracellular matrix and protect kidney through inhibiting CTGF expression and reducing TGF-p, level.关键词
益气养阴消癥通络中药/系膜细胞/细胞外基质/转化生长因子β1/结缔组织生长因子/大鼠Key words
Chinese medicinal with actions of replenishing qi and nourishing yin, and resolving mass and freeing collateral vessels/mesangial cells/extracellular matrix/transforming growth factor-?/connective tissue growth factor/rats分类
医药卫生引用本文复制引用
张江华,陈志强,赵雯红,孙玉凤,王月华,李黎莉,王凤丽,杨增芯..益气养阴消癥通络中药对高糖联合AngⅡ培养大鼠肾小球系膜细胞分泌转化生长因子β1和结缔组织生长因子的影响[J].北京中医药大学学报,2012,35(3):174-177,4.基金项目
河北省自然科学基金资助项目(No.C2008001074),河北省卫生厅资助项目(No.08165) （No.C2008001074）
