北京中医药大学学报2012，Vol.35Issue(3)：189-193,后插2,6.
黄芪和葛根素对2型糖尿病KKAy小鼠肝损害干预的实验研究
Intervention of Huangqi (Radix Astragali seu Hedysari) and puerarin to liver injury in KKAy mice with type 2 diabeles
摘要
Abstract
Objective To observe the liver injury in KKAy mice with type 2 diabetes, and study the protective effects of Huangqi ( Radix Astragali seu Hedysari) and puerarin on liver. Methods Male KKAy mice were randomly divided into model group, Huangqi group and Huangqi combining puerarin group (combining group). C57BL/J mice with the same age were selected as normal group. The change of fasting blood glucose ( FBG) was detected. The levels of triglyceride ( TG) , cholesterol (CH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected after mice were sacrificed respectively when they were 24 weeks old and 28 weeks old. The pathological sections of liver tissue were prepared and stained with HE staining and oil red 0 staining. Results In model group, the levels of FBG, blood fat, ALT and AST increased significantly than those in normal group, and there was steatosis in liver. The size of liver was larger by naked eye in model group than that in normal group and greasy by touch. There were empty lipocytes observed by microscope in different sizes in liver cells. In Huangqi group and combining group, all biochemical indexes were improved, especially the levels of AST and ALT decreased significantly, and pathological sections showed that liver structure was complete. Conclusion There are obvious liver injury and abnormal blood fat in KKAy mice, and Huangqi or Huangqi combining puerarin can effectively inhibit abnormal blood fat and protect the structure and function of liver.关键词
KKAy小鼠/肝脏损害/黄芪/葛根素Key words
KKAy mice/liver injury/Huangqi (Radix Astragali seu Hedysari) /puerarin分类
医药卫生引用本文复制引用
崔蕾,李姝玉,柴新楼,杨辉,苏玮莲,贾德贤,王谦..黄芪和葛根素对2型糖尿病KKAy小鼠肝损害干预的实验研究[J].北京中医药大学学报,2012,35(3):189-193,后插2,6.基金项目
国家自然科学基金资助项目(No.30672756 （）
81072926),北京中医药大学创新团队项目(No.2011-CXD-04) （No.2011-CXD-04）
