北京中医药大学学报2012，Vol.35Issue(4)：242-245,后插1,5.
清脑宣窍方有效组分对缺血性脑中风大鼠血脑屏障上P-糖蛋白表达的影响
Influence of effective components of Qingnao Xuanqiao Fang on expression of BBB P-glycoprotein in rats with ischemia stroke
摘要
Abstract
Objective To compare the time-series expression of P-glycoprotein (P-gp) in transportprotein in blood brain barrier (BBB) and influence of Qingnao Xuanqiao Fang on this expression in normal rats and rats with ischemia injure. Methods The rat model of ischemia stroke was established by using thread approach and then all rats were randomly divided into normal group and model group. The model group was given reperfusion at different time points (0 h, 0.5 h, 1 h, 2 h, 6 h, 12 h and 24 h). The expression of P-gp was observed in ischemia areas of cortex and hippocampus in two groups by using immunohistochemistry assay. After one day and except of sham-operation group, the rats were randomly divided into model group, high-dose, mid-dose and low-dose effective components of Qingnao Xuanqiao Fang groups (high-dose group, mid-dose group and low-dose group) , and verapamil group (Ver group) , and they were given corresponding medicinal 1 day to 5 days after modeling. After the final medicinal giving, the rats were sacrificed for collecting samples and the expression of P-gp was detected in cortex and hippocampus. Result The results of immunohistochemistry assay showed that there was P-gp positive staining observed in the ischemia areas of cortex and hippocampus in normal group and model group. The results of hemi-quantitative analysis showed that the expression of P-pg had significant difference at different time points of reperfusion, and decreased in the ischemia areas of cortex and hippocampus in model group compared with normal group (P < 0.05 ). There was significant difference in P-pg expression between model group and high-dose group, mid-dose group or low-dose group ( P < 0.05 ). The expression of P-pg decreased significantly in ischemia areas of cortex and hippocampus in high-dose group, mid-dose group, low-dose group and Ver group. Conclusion There is time-series expression of P-pg in BBB under the pathological condition of ischemia stroke, which reflects the changes of BBB permeability induced by changes of protein expression under a specific pathological status. The effective components of Qingnao Xuanqiao Fang has significant inhibitory effect on P-gp expression in the ischemia areas of cortex and hippocampus in rats with ischemia stroke.关键词
清脑宣窍方/缺血性脑中风/血脑屏障/P-糖蛋白/大鼠Key words
Qingnao Xuanqiao Fang/ischemia stroke/blood brain barrier/P-glycoprotein/rats分类
医药卫生引用本文复制引用
萨础拉,解素花,姜艳艳,李晓婷,孙建宁,石任兵..清脑宣窍方有效组分对缺血性脑中风大鼠血脑屏障上P-糖蛋白表达的影响[J].北京中医药大学学报,2012,35(4):242-245,后插1,5.基金项目
高等学校科技创新工程重大项目培育资金项目(No.706008),北京中医药大学创新团队项目(No.2011-CXTD-12),北京中医药大学自主选题项目(No.JYBZZ-XS035) （No.706008）
