北京中医药大学学报2012，Vol.35Issue(4)：284-288,5.
冠心病不稳定心绞痛血瘀证患者尿液代谢组学特征的研究
Characteristics of urine metabonomics in patients with blood stasis syndrome of CHD unstable angina
摘要
Abstract
Objective To study the changes of urine metabolites in patients with blood stasis syndrome of unstable angina of coronary heart disease ( CHD) and health volunteers by applying metabonomics method of nuclear magnetic resonance ( NMR). Methods The general materials and urine samples were collected from the patients (CHD group) and health volunteers (health group) through clinical cross-section survey, and then magnetic resonance spectrogram (MRS) was analyzed with techniques of NMR and pattern recognition technique. The spectrogram after removing water peak was treated with 0. 01ppm segmented integral and normalization. All data were imputed into SIMCA-P + software (v11. 5 , Umetrics, Sweden) and the results were analyzed by using partial least square-discriminant analysis (PLS-DA). Results The content of proline, alanine, isoleucine, glucose, valine, histidine and hippuric acid increased, and content of citric acid, creatine and taurine decreased in the urine samples of CHD group. The analysis of urine samples combined PLS-DA could significantly distinguish CHD group and health group. Conclusion The results of study showed that the changes of citric acid, proline, isoleucine and taurine in urine samples consist of the metabolites features in the patients with blood stasis syndrome of CHD unstable angina, and the study offer a new idea and method for diagnosis, treatment and TCM syndrome research.关键词
磁共振/代谢组学/冠心病不稳定心绞痛/尿液/偏最小二乘-判别分析法Key words
nuclear magnetic resonance/metabonomics/unstable angina ci coronary heart disease/urine/PLS-DA分类
医药卫生引用本文复制引用
王娟,李中峰,赵慧辉,陈建新,陈婵,柴欣楼,王伟..冠心病不稳定心绞痛血瘀证患者尿液代谢组学特征的研究[J].北京中医药大学学报,2012,35(4):284-288,5.基金项目
北京中医药大学研究生科研基金资助项目(No.2009JYBZZ-XS013) （No.2009JYBZZ-XS013）
