北京中医药大学学报2012，Vol.35Issue(5)：317-322,6.
药对蛇床子—补骨脂对乳腺癌骨转移裸鼠生存时间与骨损伤的影响
Influences of coupled medicinal-Shechuangzi (Fructus Cnidii) and Buguzhi(Fructus Psoraleae)on survival time and bone injury in nude mice with breast cancer with bone metastasis
摘要
Abstract
Objective To study the influences of coupled medicinal-Shechuangzi (Fructus Cnidii) and Buguzhi {Fructus Psorakae) on survival time, activity of osteoclast and bone injury in nude mice with breast cancer with bone metastasis, and discuss the best combination of the coupled medicinal in the treatment of breast cancer with bone metastasis. Methods The model of nude mouse was established by using left ventricle injection of MDA-MB-231BO cells. The effect of coupled medicinal-Shechuangzi and Buguzhi(2. 56 g/mouse) was observed taking zoledronic acid as a positive control medicinal. All nude mice (n =84) were randomly divided into(0.2 mg/kg) group A and group B (each n =42), and each group were randomly divided again into model group, zoledronic acid group, 4 parts Shechuangzi to 0 part Buguzhi group (4 to 0 group) , 0 part Shechuangzi to 4 parts Buguzhi group (0 to 4 group) , 1 part Shechuangzi to 3 parts Buguzhi group (1 to 3 group), 2 parts Shechuangzi to 2 parts Buguzhi group (2 to 2 group), 3 parts Shechuangzi to 1 parts Buguzhi group (3 to 1 group, each re =6). A normal group (n = 6) was additionally set into group B. The survival time was observed in group A after intervention by different proportions of medicinal. The samples were collected 6 weeks after intervention by different proportions of medicinal in group B. The changes of morphology and quantity of osteoclasts were observed after TRACP staining, and the expressions of genes and proteins of M-CSF and PTHrP were detected in bone metastasis focus. Results The survival time was significantly prolonged in all intervention groups in nude mouse model (P <0. 05, P <0. 01), and the effect was the best in 2 to 2 group, which was almost as the same as that in zoledronic acid group. The activity of osteoclasts was inhibited to some extent in all intervention groups (P < 0. 05, P < 0. 01 ) , and the effect was the best in 2 to 2 group, which was almost as the same as that in zoledronic acid group. The expressions of genes and proteins of M-CSF and PTHrP were down-regulated to some extent in all intervention groups (P <0. 05, P <0.01) , and the effect was the best in 2 to 2 group. Conclusion The coupled medicinal-Shechuangzi and Buguzhi with the action of warming kidney can inhibit breast cancer with bone metastasis, and their proportion of two to two may be the best proportion with the best curative effect.关键词
乳腺癌/骨转移/药对/蛇床子—补骨脂/小鼠Key words
breast cancer/ bone metastasis/ coupled medicinal/ Shechuangzi ( Fructus Cnidii) and Buguzhi (Fructus Psoraleae)/ mice分类
医药卫生引用本文复制引用
程旭锋,刘琦,刘胜,张新峰,杨顺芳..药对蛇床子—补骨脂对乳腺癌骨转移裸鼠生存时间与骨损伤的影响[J].北京中医药大学学报,2012,35(5):317-322,6.基金项目
国家自然科学基金项目(No.30772811),河南省教育厅自然科学基金项目(No.2010B360005),郑州市科技局科技攻关计划(No.112PPTSF319-14),郑州市金水区技术研究与开发经费支持项目(No.20093542) （No.30772811）
