北京中医药大学学报2012，Vol.35Issue(5)：323-327,5.
活血化瘀药物苏木、川芎含药血清对PG细胞增殖影响的实验研究
Influences of medicinal serum of Sumu (Lignum Sappan) and Chuanxiong(Rhizoma Ligutici)on proliferation of PG cells
摘要
Abstract
Objective To investigate the influences of medicinal serum of Sumu (Lignum Sappan) and Chuanxiong (Rhizoma Ligutki) on the proliferation of human highly metastasis PG cells cultured in normoxia and hypoxia conditions, screen the suitable concentration of medicinal serum and determine the best acting time. Methods The medicinal serum samples of Sumu and Chuanxiong were prepared and used to intervene PG cells cultured in normoxia and hypoxia conditions. The samples were then divided into 6 groups according to the concentration of medicinal serum and a control group was set. The influences of medicinal serum of Sumu and Chuanxiong on the proliferation of PG cells were observed by using MTT method. Results In normoxia condition, the differences among different concentration of medicinal serum of Sumu (except of 0.625% ) and medicinal serum of Chuanxiong ( except of 1.25% ) had statistical significance ( P < 0. 05 ). In hypoxia condition, the differences among different concentration of medicinal serum of Sumu and medicinal serum of Chuanxiong had no statistical significance (P >0.05 ). Conclusion The medicinal serum of Sumu or Chuanxiong can inhibit PG cell proliferation in normoxia condition after intervening for 24 hours and shows a dose-dependence. The higher the concentration of the serum is, the more obvious its inhibitory effect is. But in hypoxia condition, the medicinal serum can improve PG cell proliferation. With its action time increasing (48 h and 72 h) , the medicinal serum has the action of promoting PG cell proliferation ( being the most significant at 72 h) in normoxia and hypoxia conditions, and shows a dose-dependence. The higher the concentration of the serum is, the more significant PG cell proliferation is (being the most obvious in concentration of 20% ). The condition of hypoxia is more beneficial to the proliferation of PG cells promoted by the medicinal serum of Sumu or Chuanxiong.关键词
苏木/川芎/含药血清/PG/增殖/大鼠Key words
Sumu (Lignum Sappan) / Chuanxiong(Rhizoma Ligutici) / medicinal serum/ PG/ proliferation分类
医药卫生引用本文复制引用
张明辉,张培彤,任为民,周天,冯艳丽,雷锋林..活血化瘀药物苏木、川芎含药血清对PG细胞增殖影响的实验研究[J].北京中医药大学学报,2012,35(5):323-327,5.基金项目
国家自然科学基金项目(No.30873334) （No.30873334）
