北京中医药大学学报2012，Vol.35Issue(5)：343-348,6.
HPLC-DAD-MS/MS对栀子中色素成分的研究
Pigment study in fruits of Gardenia jasminoide by HPLC-DAD-MS/MS
摘要
Abstract
Objective To determine the crocins in the fruits of Gardenia jasminoides. Methods The chromatographic condition was as follows: HPLC procedure was performed on the chromatographic column of Dikma Diamonsil C18 (4.6 mm ×250 mm, 5 μm) at 30 ℃, and the mobile phase was 0.15% formic acid solution-methanol in gradient elution. The flow velocity was 0. 6 mL/min and the detection wavelength was 440 nm. The mass spectrometry condition was as follows; source of electrospray ionization (ESI) , detection of negative ion mode and scan rang being m/z = 100 - 1400. The structure of crocins was determined combining the analysis on characteristics of ultraviolet-visible absorption spectra and MS/MS dissociation. Results There were 31 crocins identified from the fruits of Gardenia jasminoides by using HPLC-DAD-MS/MS, and 23 chemical constituents deduced according to the laws of MS/MS dissociation. Conclusion HPLC-DAD-MS/MS can be used for rapid screening and identifying crocins from the fruits of Gardenia jasminoides.关键词
栀子/藏红花素类色素/HPLC-DAD-MS/MS/成分鉴定Key words
fruits of Gardenia jasminoides/ crocins/ HPLC-DAD-MS/MS/ constituent identification分类
医药卫生引用本文复制引用
高凤阳,高晓燕,张加余,张倩,乔延江,卢建秋..HPLC-DAD-MS/MS对栀子中色素成分的研究[J].北京中医药大学学报,2012,35(5):343-348,6.基金项目
国家“重大新药创制”科技重大专项项目(No.2010ZX09502-002) （No.2010ZX09502-002）
