草业科学2012，Vol.29Issue(5)：724-732,9.
丛枝菌根真菌研究中土壤灭菌方法综述
A review on methods of sterilization and inhibition of arbuscular mycorrhizal fungi in Soil
摘要
Abstract
A symbiotic relationship between arbuscular mycorrhizal fungi （AMF） and plant roots is one of the hottest studies in the microbial research field currently. Establishing a non-AMF control group （non-AMF soil） is a key point to reveal effects of arbuscular mycorrhizal fungi on plants. This paper summarizes 5 methods, including autoclaving, chemical fumigation, γ-irridation, benomyl and severance of hyphae, to establish non-arbuscular mycorrhizal fungi control groups. Autoclaving, chemical fumigation and γ-irridiation can be adopted in indoor experiments, while the former two may affect the phsical and chemical cha-rasteristics of soil and the latter is not enviromental-friendly. Benomyl and severance of hyphae are suitable for outdoor experiments. The quantity of benomyl should be rigidly controlled due to its negative effects on the environment. Though severance of hyphae would not damage the environment, it may affect the exchange of water and nutrition between plants and soil. Thus, researchers should choose suitable methods for their studies auording to enviroment and purpose of the study.关键词
丛枝菌根真菌（AMF）/高压蒸汽法/γ射线灭菌/化学熏蒸/苯菌灵/物理割断法Key words
arbuscular mycorrhizal fungi (AMF)/autoclaving/y-irradiation/chemical fumigation/benomyl/severance of hyphae分类
农业科技引用本文复制引用
谢越,杨高文,周翰舒,张英俊..丛枝菌根真菌研究中土壤灭菌方法综述[J].草业科学,2012,29(5):724-732,9.基金项目
国家自然科学基金项目 （）
牧草产业技术体系项目 （）
