草业科学2012，Vol.29Issue(5)：833-836,4.
利用菊芋一步法生产酒精工程菌构建的研究
Construction of engineered bacteria for onestep producing alcohol with Jerusalem artichoke
摘要
Abstract
To obtain fusant strains being able to produce ethanol directly from Jerusalem artichoke （Helianthus tuberosus） materials efficiently, the protoplast fusion technique was used and some appropriate conditions were chosen for protoplast preparation and fusion. The protoplast fusion was conducted between Saccharomyces cerevisiae and a penicillium strain obtained by self-screening with a high yield of inulinase. The abilities of inulinase and ethanol production of detected fusants was determined. Their genetic stability was also studied. Finally, a superior strain named R8 was chosen for inulin alcoholic fermentation. The results showed that the ethanol content of fermented products was 41.16% and the raw sugar utilization rate was 75.52%.关键词
原生质体融合/菊芋/酒精发酵/优化Key words
protoplast fusion/Jerusalem artichoke/alcoholic fermentation/optimization分类
生物科学引用本文复制引用
李雪雁,张维,张秀兰,李冰..利用菊芋一步法生产酒精工程菌构建的研究[J].草业科学,2012,29(5):833-836,4.基金项目
2009年甘肃省教育厅硕导基金项目 （）
