重庆医学2012，Vol.41Issue(23)：2358-2360,2363,4.DOI:10.3969/j.issn.1671-8348.2012.23.005
79株结核分支杆菌基因分型研究及耐药相关分析
Study on genotyping and drug-resistance analysis of 79 Mycobacterium tuberculosis
摘要
Abstract
Objective To establish the molecular characteristics of Mycobacterium tuberculosis by multiple locus variable numbers of tandem repeats(MLVA) genotyping technique and epidemiologic data for providing scientific basis to prevent and control tuberculosis.Methods M.tuberculosis clinical isolates and the patients data were collected.The polymorphism of 15 VNTR loci which were commended was tested,clustering of genotype was analyzed with BioNumerics(Version 5.0),and drug susceptibility testing was performed using proportion method.Statistical analysis was performed with Chi Square Test.Results 79 M.tuberculosis clinical isolates were analyzed respectively;the results showed that there were obvious polymorphisms of VNTRs.The discrimination power of QUB-llb,MIRU26,Mtub 21and MIRU 16 loci was high(HGI>0.7) .otherwise the MIRU 4,ETR-A and ETR-C was low(HGI <0.4).The clustering of genotype showed that these strains could be categorized into 7 gene clusters,in which the proportions of cluster Ⅲ and cluster V were the biggest,46.8% were cluster Ⅲ and 41.8% were cluster V ,other clusters were of scattered distribution.The rate of re-treated patients in the Ⅲ,V and other clusters patients was 32.43% ,21.21% and 11.11% respectively;there was no statistical significance(P>0.05).The drug resistance rate of V clusters strain(36.36%) was obviously higher than Ⅲ(10.81%) and other clusters(11.11%) ,the differences was statically significant(P<0.05).Conclusion There are obvious polymorphisms of VNTRs of M.tuberculosis clinical strains preliminarily in Mianyang area.The main epidemic strains be-longe to cluster Ⅲ and cluster V ,the main drug resistance stains belonge cluster V .It is suggested that epidemic surveillance of cluster fJI and cluster V stains,resistance surveillance of cluster V stains both needs to be strengthened.关键词
结核分支杆菌/基因分型/耐药Key words
mycobacterium tuberculosis/genotyping/drug resistance引用本文复制引用
陈志,陈佳,先德强,周云,郑幸福,江智辉..79株结核分支杆菌基因分型研究及耐药相关分析[J].重庆医学,2012,41(23):2358-2360,2363,4.基金项目
四川省卫生厅课题资助项目(080213) （080213）
绵阳市卫生局课题资助项目(200703). （200703）
