重庆医学2012，Vol.41Issue(24)：2462-2464,2468,4.DOI:10.3969/j.issn.1671-8348.2012.24.003
小鼠Boule蛋白与Crem3′UTR结合并调节其表达
Mouse boule protein binds to 3′UTR of Crem mRNA and regulates its expression
摘要
Abstract
Objective To investigate the interaction between Boule protein and sperm maturation related gene Crem and reveal the function of Boule protein in spermatogenesis.Methods The pGEX-Boule expression vector was constructed and transformed into BL21 competent cells,and induced Boule protein was purified.RT-PCR and EMSA were performed to analyze the mRNA bond to the Boule protein.Results The recombinant expression vector pGEX-Boule was successfully constructed and purified GST-Boule protein was obtained,RT-PCR and EMSA experiments indicated that Boule protein was capable to bind to Crem3 UTR.Conclusion Boule protein may regulate the expression of the Crem protein by binding to Crem3 UTR and plays a role in spermatogenesis process.关键词
EMSA/RNA结合蛋白/Boule/CremKey words
EMSA/RNA-binding protein/Boule/Crem引用本文复制引用
林昌海,曾梅,陈述,卢亦路,马用信..小鼠Boule蛋白与Crem3′UTR结合并调节其表达[J].重庆医学,2012,41(24):2462-2464,2468,4.基金项目
国家重点基础研究发展计划(973计划)基金资助项目(2012CB947600) （973计划）
国家自然科学基金资助项目(31070676) （31070676）
教育部春晖计划基金资助项目(Z2009-1-61003). （Z2009-1-61003）
