重庆医学2012，Vol.41Issue(24)：2504-2505,2509,3.DOI:10.3969/j.issn.1671-8348.2012.24.018
SOX7对胃癌MKN45细胞株体外迁移侵袭的影响
SOX7 inhibits migration and invasion of MKN45 cells in vitro
宋隆明1
作者信息
- 1. 重庆市合川区中西医结合医院外科,401520
- 折叠
摘要
Abstract
Objective To investigate the effects of SOX7 on migration and invasion of MKN45 cells in vitro.Methods SOX7 overexpression in MKN45 cells by using pIRES2-EGFP-SOX7 plasmids.Real-time PCR and Western-blot were performed to detect the mRNA level and protein level of SOX7 in MKN45 with or without SOX7 overexpression.Proliferation and invasion assay were performed to examine the changes between MKN45 cells with SOX7 expression and control.Western-blot was performed to detect the change of MMP-9 expression between MKN4 5 cells with SOX7 expression and control.Results SOX7 was significantly upreg-ulated in MKN45 after transfected.Proliferation and invasion were significantly decreased in MKN45 cells with SOX7 expression compared with control.The expression of MMP-9 was significantly decreased in MKN4 5 cells with SOX7 expression compared with control.Conclusion SOX7 could inhibit proliferation and invasion of MKN4 5 cells in vitro through blocking Wnt/β-catenin.关键词
胃癌/SOX7/MKN45/Wnt/β-catenin/侵袭转移Key words
gastric cancer/SOX7 /MKN45 /Wnt/β-catenin/invasion引用本文复制引用
宋隆明..SOX7对胃癌MKN45细胞株体外迁移侵袭的影响[J].重庆医学,2012,41(24):2504-2505,2509,3.
