摘要
Abstract
Objective To investigate the effect of IL-17 expression on ventricular remodeling with myocardial infarction(MI) in rats.Methods Liquid nitrogen cryoinjury method was used to establish myocardial infarction model.The MI models were randomly divided into 3 groups,MI control group(C group),IgG group(G group) and antibody group(A group).Then each group was randomly divided into 2 sub-groups:4 week and 8 week groups,each sub-group of 8 to 10.Kight rats of sham group underwent sham operation,being killed after 8 w(S group).The first day after operation,PBS solution were intraperitoneally injected to sham group and C group,mouse isotype IgG1 and PBS to G group,monoclonal human IL-17 antibody and PBS to A group,5 times every two days.Cardiac function and ventricular remodeling were evaluated by echocardiography and morphological examination.The protein expression of I,III collagen was assessed by the method of immunohistochemical stain.The protein expression of interleukin-17 was examined by Western blot.Results Echocardiogram demonstrated that rat models were successfully made.Compared to sham group,expression of IL-17 in myocardium in MI control group,IgG group and antibody group increased obviously at 4 weeks and 8 weeks after operation(P<0.05)and ventricular remodeling related parameters LVEDD,LVESD,EDV,ESV,LV/BW increased(P <0.05) ,LVEF,LVFS decreased(P<0.05) ;Compared to MI control group and IgG group,expression of IL-17 in antibody group was lower(P<0.05) ,and the parameters improved(P<0.05).I / HI collagen ratio were also significantly decreased in the 4 w,8 w sub-groups of Antibody group(P<0.05).While,obvious differences were not found between MI control group and IgG group.Conclusion IL-17 expression was significantly increased after MI.There is relationship between a large number of IL-17 expression and ventricular remodeling in rats with MI.关键词
心肌梗死/白介素-17(IL-17)/心室重塑Key words
myocardial infarction/interleukin-17(IL-17) /ventricular remodeling
