重庆医学2012，Vol.41Issue(26)：2692-2693,2697,封2,4.DOI:10.3969/j.issn.1671-8348.2012.26.005
硫唑嘌呤在胰腺癌动物模型制作中的应用
Application of azathioprine in preparing animal model of pancreatic cancer
摘要
Abstract
Objective To screen the azathioprine(AZA) concentration most suitable for tumorigenesis. Methods 40 SD rats were divided into 5 groups according to the AZA concentrations:blank control group(A) ,2. 6 mg/kg AZA group(B) ,2. 2 mg/kg AZA group(C) ,1. 8 mg/kg AZA group(D) and 1. 4 mg/kg AZA group(E) ,8 rats in each group. The group A was given normal saline 2mL per day and the group B,C,D and E were irrigated the corresponding concentrations of AZA 2mL. After 30 d,Mia PAC-A cell suspension liquid was subcutaneously transplanted in SD rats. Tumor formation was judged by detecting CA24-2 and preparing the pathological slice. Results On 15,20,25,30 d after inoculation of pancreatic cancer cells,blood CA24-22 levels in various model groups were increased with varying degrees. Blood CA24-2 levels on posttransplantion 15,20,25,30 d in the group B were (51. 56 ± 2. 14) ,(51. 61 ± 1. 21) ,(54. 66 ± 1. 02) ,(56. 00 ± 0. 38) U/mL respectively, showing statistical difference compared with the group C,D and E(P<0. 05). Furthermore,the group B had the highest tumor formation rate(83. 3%). In addition,the pancreatic pathological slices showed the pathological features such as the nucleus deformity,different cell size,dikaryocyte with 1-2 nucle-oli, large nucleus and deep staining. At same time, enlarged lymph nodes also found at the site of transplant and peripancreas. Conclusion AZA concentration of 2. 6 mg/kg is most conducive to prepare the rat model of pancreatic cancer.关键词
胰腺肿瘤/硫唑嘌呤/疾病模型,动物/大鼠Key words
pancreatic neoplasms/azathioprine/disease models,animal/rats引用本文复制引用
吴介恒,卢兴兵,李荣妍,吕智慧,黄莎圆子,刘梦娜,郭素红..硫唑嘌呤在胰腺癌动物模型制作中的应用[J].重庆医学,2012,41(26):2692-2693,2697,封2,4.基金项目
吉林医药学院大学生科研基金资助项目[吉医学科字(2010年)第8号]. （2010年）
