重庆医学2012，Vol.41Issue(26)：2694-2697,封2,后插1,6.DOI:10.3969/j.issn.1671-8348.2012.26.006
乳腺癌MDA-MB-231细胞源exosomes对血管内皮细胞EGFR表达的影响
Effect of exosomes derived from breast cancer cell line MDA-MB-231 on EGFR expression in HUVECs
摘要
Abstract
Objective To study the effect of exosomes derived from breast cancer cell line MDA-MB-231 on epidermal growth factor receptor(EGFR) expression in human umbilical vein endothelial cells(HUVKCs) and to research the possible manner of abnormal expression EGFR of tumor endothelial cell. Methods Exosomes were purified by serial ultracentrifugation and sugar density ultracentrifugation. The expression of KGFR in MDA-MB-231 was detected by immunocytochemistry and Western blotting. The expression of EGFR in exosomes and HUVECs was detected by Western blotting. The expression of EGFR in HUVECs with exosomes co-cultured 24 h was detected by immunocytochemistry. RT-PCR was used to detect the mRNA expression of EGFR in MDA-MB-231 and experimental group. Results MDA-MB-231 cells expressed high level of EGFR. In the results of Western blotting,the positive reaction band was found in 170 kD which coincided with the molecular weight of EGFR in MDA-MB-231 and exosomes, and negative reaction in HUVECs. When co-cultured with HUVECs and exosomes for 24 h,the result of immunocytochemistry showed that the part of the experimental group was found straw color or brown madder particle in HUVECs cytoplasm,the ratio of positive expression was 21. 4 ± 3. 1%. There was significant increase compared with the control group(P<0. 01). RT-PCR exhibited positive of EGFR mRNA in MDA-MB-231, and negative in the experimental group of HUVECs. Conclusion Exosomes derive from MDA-MB-231 cell lines with oncogene EGFR cargo,and could mediate the cargo to endothelial cell. It may be the possible manner of abnormal expression EGFR in tumor endothelial cells.关键词
乳腺肿瘤/内皮细胞/表皮生长因子/脐静脉Key words
breast neoplasms/endothelial cells/ epidermal growth factor/ umbilical veins引用本文复制引用
谢莹珊,沈宜,隆霜,范维柯,姜蓉,陈黎..乳腺癌MDA-MB-231细胞源exosomes对血管内皮细胞EGFR表达的影响[J].重庆医学,2012,41(26):2694-2697,封2,后插1,6.基金项目
重庆市渝中区科委资助项目(2009). （2009）
