大豆科学2012，Vol.31Issue(3)：347-352,357,7.
一种简便大豆原位转基因方法研究
A Simple Soybean in Planta Transformation Method
摘要
Abstract
In this paper, the terminal bud of germinated soybean seedlings were longitudinal cut and infected by Agrobacterium firstly, and then transplanted the seedlings into field. Eliminated the seedlings without branches on main stem, transformed plants leaves were painted or sprayed with 100 mg · L-1 glufosinate, 3 days later observed leaves reaction and accounted for the resistant plant number. All alive plants were identified by PCR and analyzed the expression of EPSPS gene in T2 generation. There were 75 and 65 plants with resistance to 100 mg · L-1 glufosinate in T0 and T1 generation, respectively; 6 and 52 positive plants in T0 and T1 generation by sequencing the PCR product, respectively; the result showed that EPSPS gene could inherited in offspring. This transgenic method could short transformation cycle, enhance efficacy and provide reference for the optimization and improvement of Agrobacterium transformation.关键词
大豆/根癌农杆菌/顶芽/原位转化Key words
Soybean/ Agrobacterium/Epicolyl tip/In planta transformation分类
农业科技引用本文复制引用
郭兵福,刘杰,洪慧龙,邱丽娟..一种简便大豆原位转基因方法研究[J].大豆科学,2012,31(3):347-352,357,7.基金项目
转基因生物新品种培育重大专项(2008ZX08004-001). （2008ZX08004-001）
