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吉林省龙井原位保护区野生大豆遗传多样性分析

孙晓环 刘晓冬 赵洪锟 沈波 王玉民 董英山

大豆科学2012,Vol.31Issue(3):358-363,6.
大豆科学2012,Vol.31Issue(3):358-363,6.

吉林省龙井原位保护区野生大豆遗传多样性分析

Genetic Diversity of Wild Soybean(G.soja)from Longjing in-situ Conserved Region of Jilin Province

孙晓环 1刘晓冬 2赵洪锟 1沈波 1王玉民 1董英山1

作者信息

  • 1. 吉林省农业科学院生物技术研究中心,吉林长春130033
  • 2. 黑龙江省农业科学院牡丹江分院,黑龙江牡丹江157041
  • 折叠

摘要

Abstract

Wild soybean accessions were collected from Longjing situ conserved region in Jilin province by GPS positioning. Population L,in which 90 wild soybean samples were collected (collection interval was 50 m) covered the whole in-situ conserved region. According to the natural geographical distribution,population L were divided into 3 sub-population A,B and C, respectively; meanwhile, a small sub-population B' (including 32 wild soybean samples, collection interval was 18 m)was taken from sub-population B. Clustering analysis shows that the 90 materials are mainly divided into 2 groups, and the others have a distant relationship,the distribution of the wild soybeans in Longjing conserved region was spotted,a correlation between the genetic diversity and geographical location were found among sub-populations. Simpson index ranged from 0. 3072 to 0.8393,with an average of 0.6788. Shannon-weaver index ranged from 0. 5856 to 1.9348,with a mean of 1.3663. These results indicated that there existed a highly genetic diversity in Lomgjing in-situ conserved population of Jilin province,and the genetic diversity of the three sub-populations have the same trend: A < B < C. The index of subdivision ( Fst) among the sub-populations is 0. 1419 and the gene flow(Nm)is 1.5112,indicating that 14.19% of genetic variations existed among sub-populations , and a relatively higher frequency of gene flow among the sub-populations.

关键词

野生大豆/SSR标记/遗传多样性/小种群/原位

Key words

Wild soybean/SSR marker/Genetic diversity/Sub-population/In-situ conservation.

分类

农业科技

引用本文复制引用

孙晓环,刘晓冬,赵洪锟,沈波,王玉民,董英山..吉林省龙井原位保护区野生大豆遗传多样性分析[J].大豆科学,2012,31(3):358-363,6.

基金项目

公益性行业(农业)科研专项经费资助 (农业)

农业部野生资源保护与利用项目 ()

国家自然基金青年基金项目(31000141) (31000141)

吉林省自然科学基金项目(201215198). (201215198)

大豆科学

OA北大核心CSCDCSTPCD

1000-9841

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