大豆科学2012，Vol.31Issue(3)：364-367,373,5.
大豆子叶节和胚尖再生体系的比较及大豆SR1基因的遗传转化
Comparison with Cotyledonary Node and Embryonic Tip Regeneration System in Soybean[Glycine max(L.)Merrill] and Genetic Transformation of SR1
摘要
Abstract
In this study,the soybean cotyledonary node and embryonic tip were used as explants to transform SRI gene into Hefeng 25 and Hefeng 35, which are susceptible to soybean Phytophthora root rot. The results showed that the regeneration of Hefeng 35 reached 96.1% and 79.1% ,in embryonic tip and cotyledon node system,respectively,which was significantly higher than that of Hefeng 25 ( 66.45 % and 74.4% ). The mean regeneration period of Hefeng 35 in embryonic tip and cotyledonary node transformation system was 40 and 68 d, respectively. When the explants of the two systems grew on the SIM or SEM for 20 days,the regeneration frequency of embryonic tip(96. 1% )was higher than that in cotyledonary node transformation sys-tem(77. 8% ). The SRI gene was transferred into Hefeng 35 by embryonic tip explants,and obtained 6 transgenic plants.关键词
大豆/子叶节/胚尖/SR1/遗传转化Key words
Soybean/Cotyledonary node/Embryonic tip/SRI/Genetic transformation分类
农业科技引用本文复制引用
姚丙晨,沈艳茹,韩雪,王琳琳,刘春燕,胡国华,陈庆山..大豆子叶节和胚尖再生体系的比较及大豆SR1基因的遗传转化[J].大豆科学,2012,31(3):364-367,373,5.基金项目
转基因专项(2009ZX08009-013B) （2009ZX08009-013B）
公益性行业(农业)科研专项(200903003). （农业）
