大豆科学2012，Vol.31Issue(3)：425-430,6.
连作与轮作大豆土壤反硝化细菌多样性与组成结构
Molecular Deversity and Characterization of Nitrite Reductase Genes from Continuous and Rotational Cropping Soybean
肖翠红 1孙冬梅 1汤晖 2刘振华1
作者信息
- 1. 黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319
- 2. 河北大学生命科学技术学院,河北保定071002
- 折叠
摘要
Abstract
Gene sequence analysis of nitrite reductases was performed on continuous cropping and rotational cropping soils of soybean to assess their incidence in different bacterial taxa and their taxonomical value.NirS gene fragments could be amplified from both soils.A total of 268 nirS clones were detected by restriction fragment length polymorphism(RFLP) analysis and obtained 29 and 34 distinct nirS clones in continuous cropping and rotational cropping soils respectively.A dominant nirS pattern,a single dominant nirS restriction pattern of rotational cropping,was observed in both samples.Beside above pattern,another two dominant patterns were also detected in continuous cropping soil.Many of the sequences belonged to dominant pattern were not closely related to previously observed genes and some phylogenetically related sequences were obtained from similar samples.The results indicated that the continuous cropping and rotational cropping soils contained novel nirS sequences,functional diversity of nirS genes changed in the two type soils.关键词
大豆/连作/轮作/亚硝酸盐还原酶Key words
Continuous cropping/ Soybean/ Rotational cropping/ Nitrite reductase (nirS )分类
农业科技引用本文复制引用
肖翠红,孙冬梅,汤晖,刘振华..连作与轮作大豆土壤反硝化细菌多样性与组成结构[J].大豆科学,2012,31(3):425-430,6.
