中德临床肿瘤学杂志（英文版）2012，Vol.11Issue(9)：522-526,5.DOI:10.1007/s10330-012-1028-3
选择性环氧合酶-2抑制剂NS-398对胆管癌细胞件QBC939的抑制作用
The inhibitory effect of selective cyclooxygenase-2 inhibitor NS-398 on the cholangiocarcinoma line (QBC939)
Youjie Fan 1Jingyu Cao 1Wende Sun 1Rui Han 1Shihai Liu1
作者信息
- 1. Department of Hepatobiliary Surgery, The Affiliated Hospital, Qingdao University Medical College, Qingdao 266003,China
- 折叠
摘要
Abstract
Objective: We studied the inhibitory effect of human cholangiocarcinoma line (QBC939) treated by selective cyclooxygenase-2 (COX-2) inhibitor NS-398 and provided the theoretical foundation for the clinical practice. Methods: Selective COX-2 inhibitor NS-398 on the growth suppression was evaluated by MTT method. The apoptotic rate was quantified and cell cycle by flow cytometry (FCM). Invasive ability was detected by Transwell. The expression of vascular endothelial growth factor (VEGF) and COX-2 in cholangiocarcinoma line was determined by enzyme-linked immunosorbent assay (ELISA).Results: NS-398 could be time-dose dependently inhibited the growth, induced apoptosis, invasived ability, S phase was inhibited, down-regulate the expression of VEGF and COX-2 in cholangiocarcinoma line (QBC939). Conclusion: NS398 can inhibit proliferation of cholangiocarcinoma cell Iine QBC939 and inhibit the invasive ability and induce its apoptosis in vitro,which may contributed to the COX-2 dependent pathway to reduce the release of VEGF.关键词
NS-398/COX-2/vascular endothelial growth factor (VEGF)/cholangiocarcinomaKey words
NS-398/COX-2/vascular endothelial growth factor (VEGF)/cholangiocarcinoma引用本文复制引用
Youjie Fan,Jingyu Cao,Wende Sun,Rui Han,Shihai Liu..选择性环氧合酶-2抑制剂NS-398对胆管癌细胞件QBC939的抑制作用[J].中德临床肿瘤学杂志（英文版）,2012,11(9):522-526,5.
