大连工业大学学报2012，Vol.31Issue(3)：177-180,4.
响应面法优化芽孢杆菌LJ-7发酵产酯酶条件
Optimization of fermentation conditions for Bacillus sp.L J-7 esterase by response surface methodology
齐勇 1王际辉 1叶淑红 1王晗 1陈富远2
作者信息
- 1. 大连工业大学辽宁省食品生物技术重点实验室,辽宁大连 116034
- 2. 大连础明集团有限公司,辽宁大连 116021
- 折叠
摘要
Abstract
In this manuscript, response surface methodology was used in order to optimize the fermentation conditions of Bacillus sp. LJ-7 esterase. The three factors with prominent influence on yield of esterase, fermentation time, fermentation temperature and initial pH value of medium, were determined through single-factor experiments. The Box-Benhnken central composite experiments were applied to further determine the optimal fermentation conditions, which were based on the results of single-factor experiments. And the optimal fermentation conditions were described as follow: fermentation time was 42.81 h, fermentation temperature was 29.40 ℃ , initial pH value of medium was 6.21, respectively. Under the optimal conditions, the anticipant esterase activity was 24. 91 U/mL, while the practical esterase activity was 24. 63 U/ML up to 95% of the anticipant value. The model has better prediction and great applicable value in esterase fermentation.关键词
发酵/响应面法/酯酶/芽孢杆菌Key words
fermentation/ response surface methodology/ esterase/ Bacillus分类
轻工纺织引用本文复制引用
齐勇,王际辉,叶淑红,王晗,陈富远..响应面法优化芽孢杆菌LJ-7发酵产酯酶条件[J].大连工业大学学报,2012,31(3):177-180,4.
