南方医科大学学报2012，Vol.32Issue(6)：766-771,6.DOI:10.3969/j.issn.1673-4254.2012.06.003
衣霉素联合顺铂对人鼻咽癌细胞增殖和凋亡的影响
Effect of tunicamycin combined with cisplatin on proliferation and apoptosis of human nasopharyngeal carcinoma cells in vitro
摘要
Abstract
Objective To study the effects of tunicamycin (a glycosylation inhibitor) combined with cisplatin on the proliferation and apoptosis of human nasopharyngeal carcinoma cells and explore the molecular mechanism.Methods Nasopharyngeal carcinoma CNE-1 and CNE-2 cells cultured in vitro were treated with different concentrations of tunicamycin with or without cisplatin.The inhibition of cell proliferation was examined using MTT assay and colony formation assay,and the cell apoptosis was analyzed using flow cytometry with propidium iodide staining.The expressions of Bax,Bd-2,and GRP78 in cells treated with 3 umol/L tunicamycin with or without 6.00 umol/L cisplatin were measured with Western blotting.Results Treatment with tunicamycin or cisplatin obviously inhibited the proliferation of CNE-1 and CNE-2 cells.Treatment with 3 umol/L tunicamycin for 24,36 and 48 h resulted in a viability of 72.13%,51.97%,and 37.56% in CNE-1 cells and 85.61%,56.95%,and 43.66% in CNE-2 cells,respectively,and the combined treatment with 6 μmol/L cisplatin lowered the cell viability to 67.97%,47.76%,and 34.68% in CNE-1 cells and 56.89%,37.05%,and 29.30% in CNE-2 cells,respectively.Tunicamycin at 0.3 umol/L combined with 0.6 μmol/L dsplatin showed an obviously enhanced inhibitory effect on colony formation of CNE-1 and CNE-2 cells.Tunicamycin treatment (3 umol/L) of CNE-1 and CNE-2 cells for 48 h induced an apoptosis rate of only 8.89% and 8.67%,but when combined with 6 umol/L cisplatin,the cell apoptosis rate increased to 37.02% and 32.25%,significantly higher than that in cells with cisplatin treatment alone (7.25% and 6.36%,respectively).Compared with tunicamycin and cisplatin alone,the combined treatment significantly increased Bax expression and decreased Bd-2 expression in the cells;tunicamycin up-regulated the expression of GRP-78 and enhanced the activity of caspase-3.Conclusion Tunicamycin can inhibit the proliferation of CNE-1 and CNE-2 cells and enhance dsplatin-induced cell death,the mechanism of which may involve excessive endoplasmic reticulum stress response and increased activity of caspase-3.关键词
鼻咽癌/衣霉素/顺铂/内质网应激/葡萄糖调节蛋白78/半胱氨酸天冬氨酸蛋白酶3Key words
nasopharyngeal carcinoma/tunicamycin/dsplatin/ERS/GRP-78/caspase-3分类
医药卫生引用本文复制引用
宋乐乐,马琳艳,张旭东,蒋志文,刘浩,蒋琛琛..衣霉素联合顺铂对人鼻咽癌细胞增殖和凋亡的影响[J].南方医科大学学报,2012,32(6):766-771,6.基金项目
国家自然科学基金(81000992,81072207) （81000992,81072207）
安徽省高校省级自然科学研究重点项目(KJ2012A202) （KJ2012A202）
安徽省自然科学基金(090413135) （090413135）
