南方医科大学学报2012，Vol.32Issue(6)：830-834,5.DOI:10.3969/j.issn.1673-4254.2012.06.016
乌司他丁和胸腺肽α1联合治疗严重脓毒症的组织病理学观察
Ulinastatin combined with thymosin α1 for treatment of severe sepsis: a histopathological observation in rats
摘要
Abstract
Objective To evaluate the therapeutic effect of ulinastatin combined with thymosin al in the treatment of severe sepsis in rats.Methods Normal Wistar rats were subject to cecal ligation and puncture (CLP) to establish models of severe sepsis.The rats were then randomized into 4 groups for treatment with saline (control),ulinastatin,thymosin al,or the combination of the latter two injected through the caudal vein or subcutaqneously at 6,24,48 and 72 h after modeling.The mortality rate was recorded daily and the rats were executed at 24,48,72 and 96 h after CLP to harvest the heart,liver,spleen,lung,kidney and small intestines for pathological examination.The spleen of the rats were taken for detection of apoptosis of the spleen cells.Results The mortality rate of the septic rats in the combined treatment group was decreased significantly (P= 0.0325).The control group showed the most severe organ damage,which was moderate in single drug treatment group and the mildest in combined treatment group.Obvious spleen cell apoptosis was found in the control group,and was significantly ameliorated in the combined treatment group [(47.4+10.9)% vs (39.3±11.4)%,P=0.0000].Conclusion Combined treatment with ulinastatin and thymosin al can significantly improve the prognosis and ameliorate organ damage and spleen cell apoptosis in rats with sever sepsis.关键词
脓毒症/病理学/乌司他丁/胸腺肽α1/联合用药Key words
sepsis/pathology/ulinastatin/thymosin al/combination therapy分类
医药卫生引用本文复制引用
郭剑颖,邓群,郭旭升,刘双庆,张玉红,何忠杰,林洪远..乌司他丁和胸腺肽α1联合治疗严重脓毒症的组织病理学观察[J].南方医科大学学报,2012,32(6):830-834,5.基金项目
北京市科技计划重大项目 （）
