南方医科大学学报2012，Vol.32Issue(6)：913-915,3.DOI:10.3969/j.issn.1673-4254.2012.06.037
甲状腺激素转运体MCT8在2VO大鼠脑组织中的表达
Expression of monocarboxylate transporter 8 mRNA in the brain tissue of rats with cerebral ischemia
摘要
Abstract
Objective To investigate the mRNA expression of monocarboxylate transporter 8 (MCT8),a thyroid hormone transport protein,in the lateral ventricle of rats with cerebral ischemia.Methods Immunofluorescence staining was used to observe the expression of MCT8 in the lateral ventricle of 5 normal SD rats.Another 20 adult male SD rats were randomized into 4 groups and subject to permanent ligation of both the common carotid arteries (2-vessel occlusion,2VO) for 3 days,2 weeks,or 5 weeks,or no ligation (control).At the end of the experiment,the transcriptional level of MCT8 in the brain tissue of the rats were detected using fluorescent quantitative PCR.Results MCT8 mRNA levels in 3-day and 2-week 2VO groups were comparable with that in the control group (P=0.909;P=0.694),but increased significantly in 5-week 2VO group compared with that in the control and 3-day 2VO groups (P=0.029;P=0.023).No significance was found in MCT8 mRNA between the 2-week and 5-week 2VO groups (P=0.065).Conclusion Prolonged cerebral ischemia causes compensatory increase of MCT8 mRNA expression on the capillary endothelial cell membranes in the lateral ventricle of rats.关键词
脑缺血/单羧酸转运蛋白8/甲状腺激素Key words
cerebral ischemia/monocarboxylate transporter 8/thyroid hormone分类
医药卫生引用本文复制引用
崔丹,管玉青,蒋海山,汪槿,席蕾,王群..甲状腺激素转运体MCT8在2VO大鼠脑组织中的表达[J].南方医科大学学报,2012,32(6):913-915,3.基金项目
南方医院院长基金(2010Z011) （2010Z011）
