辐射防护2012，Vol.32Issue(4)：199-203,209,6.
不同剂量电离辐射对小鼠树突状细胞（DC）表型及功能的影响
Effect of Different Doses of Ionizing Radiation on Phenotype and Function of Dendritic Cells
摘要
Abstract
To study the effect of ionizing radiation on Dendritic Cells （DC）, Flow Cytometry （FCM） and Enzyme-linked Immune-sorbent Assay （ELISA） were used to detect the expression of co- stimulatory molecules CD40 and CD80 on DC as well as their secretion of IL-12 and IL-27 in DC/TLC coculture system after having been exposed to 0.075 Gy and 2.0 Gy X-rays for 6 h, 12 h, 24 h, 48 h and 72 h respectively. The results demonstrated that 0.075 Gy X-rays can induce CD80 and CD40 on DC and stimulate IL-12 secretion, while they were suppressed by 2.0 Gy. However, IL-27 secretion by DC was stimulated after both low and high doses of radiation. These results suggested that ionizing radiation can cause changes of the phenotype and secretion of cytokines, and this will provide new scientific basis for the study of immune effect following ionizing irradiation.关键词
X射线/树突状细胞/细胞表型/白介素-12/白介素-27Key words
X-rays/dendritic cells/cellular phenotype/interleukin-12/interleukin-27分类
医药卫生引用本文复制引用
董娟聪,王金虎,卢良杰,金顺子..不同剂量电离辐射对小鼠树突状细胞（DC）表型及功能的影响[J].辐射防护,2012,32(4):199-203,209,6.基金项目
基金项目：国家自然科学基金资助项目（30870584）. （）
