辐射防护2012，Vol.32Issue(4)：204-209,6.
氚β辐射对神经细胞迁移相关因子表达的影响
Effects of Tritium β Irradiation on the Expression of Regulated Factors Associated with the Neural Cell Migration
摘要
Abstract
To explore the effects of HTO β irradiation on neural cell migration primary neural ceils were exposed to β rays from 0, 3.7 ×10^3, 3.7 ×10^4, 3.7 ×10^5 and 3.7 ×10^6 Bq/mL HTO for 24 h respectively., The migration distance of neural cells was measured with Leica AF 6000 live cell workstation, [Ca^2＋] in neurons were detected using Fluo-3-AM fluorescent probe and flow cytometry, and the expressions of β-tubulin and NCAM proteins in neurons were assayed by Western blot. Results showed that： with the augmentation of HTO concentration and the increase of β irradiation doses, the distance of neural cells migration accordingly was reduced, intracellular free calcium ions in neurons were incremented, and the expressions of ~-tubulin and NCAM protein also were decreased. It suggested that HTO β irradiation may cause the alterations of the regulated factors associated with neural cell migration to prevent neural cell from migrating.关键词
氚β辐射/神经细胞迁移/钙离子/β-tubulin/NCAMKey words
HTO β irradiation/neural cell migration/Ca^2+/β-tubulin/NCAM分类
生物科学引用本文复制引用
邱俊,蔡二朋,吴翠萍,王明明..氚β辐射对神经细胞迁移相关因子表达的影响[J].辐射防护,2012,32(4):204-209,6.基金项目
基金项目：安徽省教育厅自然科学基金资助项目（KJ20112162）,中科院新型薄膜太阳电池重点实验室开放基金资助项目（2010007）,安徽高校省级教学质量工程项目（20101941）. （）
