辐射防护2012，Vol.32Issue(4)：210-214,5.
安多霖对高功率微波照射大鼠睾丸细胞凋亡相关蛋白的影响
The Effect of Anduolin on Apoptosis-related Protein of Testicular Cells in Rats Irradiated by High-power Microwave
郝述霞 1王春燕 1苟巧 1齐雪松 1张翠兰 1佟鹏 1张伟 1吕慧敏1
作者信息
- 1. 中国疾病预防控制中心辐射防护与核安全医学所,北京100088
- 折叠
摘要
Abstract
To observe the change of testicular apoptosis related proteins as p53, cyt-c and apaf-1 in rats irradiated by high power microwave, sexually matured Wistar rats were randomly divided into 5 groups： control group, model group, 3 mg/kg, 6 mg/kg and 9 mg/kg treated group. The rats of treated groups were given drug continuously by oral administration for 7 days, and the control group and model group were given distilled water every day. Then, treated groups and model group were irradiated with the high-power microwave of 100 roW/era2 for 15 rain. Rats were killed before irradiation and 3 day, 7 day and 10 day after irradiation respectively and testes were fixed. Interested proteins in testicular tissue were detected with Immunohistoehemical method, the protein content were analyzed with Image pro plus analysis software then. The results showed that the protein content of p53 in the testicular cell of model group was significantly lower than that of the control group （p 〈0.05） at 3 day, 7 day and 10 day after irradiation, but the treated group were significantly higher than the model group （p 〈 0.05） ; cyt-e content in rats of model group was significantly lower than that of the control group （p〈 0.05） , also the cyt-c content in treated groups but still significantly higher than model group （p 〈 0.05） ; as for the apaf-1, the content of the model group was significantly higher than the control group （p〈0.05） , while the treatment group were significantly lower than the model group （p〈0.05）关键词
高功率微波/安多霖/凋亡/p53/cyt-c/apaf-1Key words
Anduolin/high-power microwave/apoptosis/protein 53/cytochrome C/apoptotic protease activating factor-1分类
生物科学引用本文复制引用
郝述霞,王春燕,苟巧,齐雪松,张翠兰,佟鹏,张伟,吕慧敏..安多霖对高功率微波照射大鼠睾丸细胞凋亡相关蛋白的影响[J].辐射防护,2012,32(4):210-214,5.
