分析化学2012，Vol.40Issue(7)：1019-1024,6.DOI:10.3724/SP.J.1096.2012.10791
基于固相萃取的微量糖蛋白糖基化修饰表征技术的建立
Characterization of N-Glycosylation Status of Micro-level Glycoprotein Using Solid Phase Extraction Based Techniques
摘要
Abstract
A new stratcgy integrating the latest hydrophilic interaction chromatographic (HILIC) method with mass spectrometry technologies was developed for the enrichment and determination of intact glycopeptides and glycan structures from a glycoprotein. The average molecular weight, the ratio of glycosylation, the glycosite and the structures of glycoforms of ribonuclease B (RNascB) were determined by MALDI-TOF-MS and ESI-LTQ-FT-MS. RNaseB contains 8. 43% glycan and has a single glycosylation site at Asn34 with the glycan structure varied from five to nine mannose residues (Man5-9GlcNAc2). The strategy paves the way for systematic and confident analysis of complex glycoproteins with important biological or pharmaceutical functions.关键词
糖蛋白/亲水固相萃取/基质辅助激光解吸电离飞行时间质谱/线性离子阱傅里叶变换离子回旋共振质谱仪Key words
Glycoprotein/ Hydrophilic interaction chromatographic solid phase extraction/ Matri-xassisted laser desorption/ionization-time of flight-mass spectrometry/ Linear quadrupole ion trap-Fourier transform ion cyclotron resonance mass spectrometry引用本文复制引用
江静,韩欢欢,马成,王继峰,应万涛,钱小红..基于固相萃取的微量糖蛋白糖基化修饰表征技术的建立[J].分析化学,2012,40(7):1019-1024,6.基金项目
本文系国家973课题(No.2011CB910603)、国家自然科学基金(No.2073505,30900258)和重点实验室自主研究课题(No.SKLP-K200902)资助 （No.2011CB910603）
