分析化学2012，Vol.40Issue(7)：1059-1064,6.DOI:10.3724/SP.J.1096.2012.11310
分散固相萃取-超高压液相色谱-串联质谱法测定茶叶中赤霉酸和α-萘乙酸
Determination of Gibberellic Acid and a-Naphthlene Acetic Acid in Tea by Ultra-pressure Liquid Chromatography Tandem Mass Spectrometry with Dispersive Solid Phase Extraction
摘要
Abstract
A rapid analytical method based on ultra-pressure liquid chromatography tandem mass has been developed for the determination of plant hormones gibberellic acid (GA3) and α-naphthlene acetic acid (NAA) in tea. The analytes were extracted from the homogenizated sample using methonolic, cleaned-up with mixture of four sorbents Florisil. graphitized carbon black (GCB), primary secondary amine (PSA) and C18. GA3 and NAA were separated on HSS C18 column (100 mm ×2. 0 mm, 1. 8 μrn) and determined on UPLC-MS/MS by negative-electrospray ionization (ESI") using multiple reaction monitoring (MRM). Quantification was done by the external standard curves. The linear range of GA3 and NAA was 0. 05 - 5. 0 mg/kg and 0. 10 -5. 0 mg/kg respectively, the correlation coefficient (r) of both plant hormone standard curve was more than or equal to 0. 9990, the limit of quantification of GA3 was 0. 05 mg/kg and that of NAA was 0. 10 mg/kg. The recoveries of three levels spiked with 0. 1, 0. 5 and 1. 0 mg/kg were 85. 0%-86. 8% for GA3 and 82. 9%-84.4% for NAA, the relative standard deviations of both plant hormones were less than or equal to 4. 5%. The method is simple, accurate and sensitive for the simultaneous determination of GA3 and NAA residual in tea.关键词
赤霉酸/α-萘乙酸/分散固相萃取/超高压液相色谱串联质谱Key words
Gibberellie acid/α-Naphthlene acetic acid/ Dispersive solid phase extraction,Ultra-pressure liquid chromatography tandem mass spectrometry引用本文复制引用
陈红平,刘新,王川丕,尹鹏..分散固相萃取-超高压液相色谱-串联质谱法测定茶叶中赤霉酸和α-萘乙酸[J].分析化学,2012,40(7):1059-1064,6.基金项目
本文系现代农业产业技术体系建设专项基金(No.nycytx-26)和浙江省分析测试科技计划项目(No.2011C37037)资助 （No.nycytx-26）
