分析化学2012，Vol.40Issue(7)：1081-1085,5.DOI:10.3724/SP.J.1096.2012.11306
解吸附电晕束电离质谱法快速筛选保健食品中非法添加的5型磷酸二酯酶抑制剂
Rapid Determination of Illegally Added Phonphodiesterase 5 Inhibitors in Health Food by Desorption Corona Beam Ionization-Tandem Mass Spectrometry
摘要
Abstract
A rapid method using desorption corona beam ionization-ion trap mass spectrometry (DCBI-MS) was developed for the determination of three phosphodiesterase 5 (PDE5) inhibitors, i. e., vardenafii, sildenafil, tadalafil in health food. The MS spectra and MS2 spectra of the inhibitors were used for prescreening and confirmation respectively. The characteristic ions of the PDE5 inhibitors were chosen for semi-quantitative. Precise quantification of the PDE5 inhibitors was carried out by high performance liquid chromatography-uitraviolet (HPLC-UV). Vardenafii, siidenafil and tadalafil were detected in one sample, three samples, and seven samples, respectively. These results were in accordance with those obtained by HPLC-UV. The fragmentation pathways of the PDE5 inhibitors were studied and based on it one sample was figured out to contain hydroxyhomosidenafil. The DCBI-MS method is rapid and accurate, which had more advantages to screen the illegally added PDE5 inhibitors in samples with complex matrix and the semiquantitative results could provide basis for HPLC analysis.关键词
解吸附电晕束离子化/离子阱质谱/高效液相色谱-紫外检测法/磷酸二酯酶5抑制剂/裂解机理Key words
Desorption corona beam ionizationt Ion trap mass spectrometry/ High performance liquid chromatography-uitraviolet detection/ Phosphodiesterase 5 inhibitors/ Fragmentation pathways引用本文复制引用
吴双,王华,朱慧果,赵勇,田清青,孙文剑,丁力,陈波..解吸附电晕束电离质谱法快速筛选保健食品中非法添加的5型磷酸二酯酶抑制剂[J].分析化学,2012,40(7):1081-1085,5.基金项目
本文系国家“863”课题(No.2010AA023001),国家自然科学基金(No.20927005),湖南省科技计划课题(No.2010TT1001)资助项目 （No.2010AA023001）
