分析化学2012，Vol.40Issue(7)：1086-1091,6.DOI:10.3724/SP.J.1096.2012.11097
离子液体保护聚苯胺纳米金复合膜传感器的制备及其在检测乳制品中金黄色葡萄球菌毒素B的应用
An Electrochemical Immunosensor Modified with Polyaniline-Gold Composite Film for Detecting Staphylococcus Aureus Enterotoxin B in Dairy Products
摘要
Abstract
Staphylococcus aureus enterotoxin B (SEB) is a kind of extracellular toxins that is secreted by Staphylococcus aureus and mainly existed in high protein foods, for example, milk. In this study, an immunosensor modified with polyaniline-gold composite film was prepared and its characteristics such as favorable conductivity of the material, high stability due to the protective effect of ionic liquid and lower detection limit were also investigated. The sensor was characterized and detected by cyclic voltammetry and electrochemical impedance spectroscopy in the Fe(CN)63-/4- solution. The relevant conditions was optimized, and the SEB standard curve, Y=933. 46χ + 1399. 8, was established. The linear range was 0. 1 - 8μg/L with a correlation coefficient of 0. 9932, and the detection limit was 0.033 μg/L (S/N = 3). The sensor showed good specificity, dairy detection rate between 88% -119%, renewable use, stability, and has been used for the rapid detection of SEB in dairy products.关键词
免疫传感器/乳制品/金黄色葡萄球菌肠毒素B/聚苯胺纳米金修饰膜/交流阻抗法/离子液体Key words
Immunosensor/ Dairy product/ Staphylococcus aureus enterotoxin B/ Polyaniline-gold composite film/ Electrochemical impedance spectroscopy/ Ionic liquid引用本文复制引用
孙秀兰,高博,张银志..离子液体保护聚苯胺纳米金复合膜传感器的制备及其在检测乳制品中金黄色葡萄球菌毒素B的应用[J].分析化学,2012,40(7):1086-1091,6.基金项目
本文系国家自然科学基金项目(No.20806033),张家港市科技局项目(No.ZKS0904),中央高校基本科研业务费专项资金项目(No.JUSRP21127)资助 （No.20806033）
