分析化学2012，Vol.40Issue(7)：1114-1117,4.DOI:10.3724/SP.J.1096.2012.11081
时间分辨荧光免疫分析方法检测烟草中菌核净残留
Determination of Dimethachlon in Tobacco by Time-resolved Fluorescence Immunoassay
摘要
Abstract
A sensitive time-resolved fluorescence immunoassay (TRFIA) with a cleanup-free pretreat-ment for dimethachlon in tobacco was developed The competitive indirect TRFIA for dimethachlon was established by using goat anti-rabbit IgG conjugated with a Eu-N1 delate as a tracer. The detection limit ( I10) and I50 for this assay was 1. 98 μg/L and 32. 00 μg/L, respectively. And the linear range for detection is between 3. 97 -128. 76 μg/L. Meanwhile, the effect of tobacco matrix to TRFIA was also studied. It was demonstrated that the linear range of inhibition curve with 1 % tobacco matrix was parallel to the standard curve; so the extract of tobacco would be diluted 200 times before assay, the index of matrix interference (Im) was calculated as 17. 1. Three levels of dimethachon (1,7 and 24 mg/L) were spiked into tobacco, the overall recoveries and the relative standard deviations (RSDs) were in the range of 73%-128% and 4. 3 -13. 2, and the practical detection limit for the method was 1 mg/L. This assay is a suitable tool for screening dimethachlon residue in tobacco samples关键词
菌核净/时间分辨荧光免疫分析/烟草/样品前处理Key words
Dimethachlon/Time-resolved fluorescence immunoassay/ Tobacco/ Sample pretreatment引用本文复制引用
刘媛,刘蓉蓉,刘贤金,方敦煌,宋春满,Huovinen Tuomas,Vehni(a)inen Markus,L(o)vgren Timo..时间分辨荧光免疫分析方法检测烟草中菌核净残留[J].分析化学,2012,40(7):1114-1117,4.基金项目
本文系国家自然科学基金项目(No.30871658)和农业部948项目(Nos.2011-Z46,2011-G5-7)资助 （No.30871658）
