法医学杂志2012，Vol.28Issue(3)：175-178,4.DOI:10.3969/j.issn.1004-5619.2012.03.004
前列腺素D2、羧肽酶A3和血小板活化因子在过敏性休克豚鼠体内含量变化
Changes of Prostaglandin D2,Carboxypeptidase A3 and Platelet Activating Factor in Guinea Pig in Anaphylactic Shock
摘要
Abstract
Objective To detect the changes of leukotriene E4(LTE4), prostaglandin D2(PGD2), earboxypep-tidase A3(CPA3) and platelet activating factor(PAF) in guinea pigs died from anaphylactic shock. Methods Guinea pigs were used for establishing anaphylactic shock models. The levels of LTE4, PGD2 and CPA3, and PAF were detected in urine, plasma, and brain tissues with ELISA kit, respectively. The significant biomarkers were selected comparing with control group. The changes of PGD2, CPA3 and PAF in the guinea pigs at time zero, 12 and 24 hours after death were observed and compared respectively. The effect of platelet activating factor acetylhydrolase(PAF-AH) to PAF in guinea pig brain was examined and compared. Results There were no statistically differences of LTE4 levels in urine observed between experimental group and control group. The levels of CPA3, PGD2 and PAF in the experimental group were significantly higher than that in the control group at Oh. The levels of PAF at 12 and 24 hours after anaphylactic shock were significantly higher than that in the control group. The levels of PAF decreased significantly after pretreatment with PAF-AH. Conclusion LTE4 in urine cannot be selected as a biomarker to determine the anaphylactic shock. PGD2 and CPA3 in plasma, and PAF in brain tissue may be used as biomarkers to determine the anaphylactic shock. PAF-AH may be potentially useful for clinical treatment of anaphylactic shock.关键词
法医病理学/过敏反应/白三烯E4/前列腺素D2/羧肽酶A3/血小板活化因子/豚鼠Key words
forensic pathology/ anaphylaxis/ leukotriene E4/ prostaglandin D2/ carhnxypeptidase A3/ platelet activating factor/ guinea pigs分类
社会科学引用本文复制引用
杨凯,郭相杰,闫学斌,高彩荣..前列腺素D2、羧肽酶A3和血小板活化因子在过敏性休克豚鼠体内含量变化[J].法医学杂志,2012,28(3):175-178,4.基金项目
山西省回国留学人员科研资助项目(2009-51) （2009-51）
山西省国际科技合作项目(2010081063) （2010081063）
