法医学杂志2012，Vol.28Issue(3)：190-194,5.DOI:10.3969/j.issn.1004-5619.2012.03.008
利用STR和Y染色体二等位基因标记鉴定同胞兄弟关系
Identification of Sibling Brothers Using STR and Y-biallelic Markers
汤美云 1黄健 1蔡金洪 1黄晓松 1徐玮 2瞿志雄 1单飞豹 1陆惠玲3
作者信息
- 1. 湖南省第二人民医院芙蓉司法鉴定中心,湖南长沙410007
- 2. 湖南省人民医院临床医学研究所,湖南长沙410007
- 3. 中山大学中山医学院法医物证学教研室,广东广州510089
- 折叠
摘要
Abstract
Objective To explore the methods for identification of sibling brothers with Y-STR locus mutation by detection of genetic markers on autosome and Y-biallelic. Methods Goldeneye 20A and 18NC kit were used to genotyped the 35 STRs on autosome from two men. PowerPlex? Y kit and Yfiler kit were used to genotyped the 16 STRs on Y chromosome full sibling index was calculated by ITO method. Twenty Y-biallelic markers were genotyped by fragment length discrepant allele specific PCR or general PCR. Results Relationship of sibling brothers was found to have mutation of 2 loci on 16 Y-STR and the identical genetype of 20 Y-biallelic markers as well as a cumulative full sibling index of 4.3149×106 from 35 STRs on autosome. Conclusion In identification of paternal linage of Y-STR mutation, more genetic information can be acquired by detection of Y-biallelic markers including SNP and InDel.关键词
法医遗传学/同胞/染色体,人,Y/STR/突变/二等位基因Key words
forensic genetics/ siblings/ chromosomes, human, Y/ STR/ mutation/ biallelic分类
社会科学引用本文复制引用
汤美云,黄健,蔡金洪,黄晓松,徐玮,瞿志雄,单飞豹,陆惠玲..利用STR和Y染色体二等位基因标记鉴定同胞兄弟关系[J].法医学杂志,2012,28(3):190-194,5.
