广东牙病防治2012，Vol.20Issue(6)：285-287,3.
3种特殊染色法显示种植体骨磨片组织学效果比较
Comparison of histological effect in section with implant by different methods of staining
摘要
Abstract
Objective To evaluate a better histological method for distinguishing the histological differences of implant-bone interface. Three different staining methods were used to stain undecalcified ground section with implant. Methods Undecalcified ground sections with implant were collected which has been buried in tibia of Beagle dog for 8 weeks. The sections were stained by three different methods respectively: methylene blue-acid fuchsin, Goldner's tri-chrome and toluidine. Microscope analysis has been performed on the implant-bone interface of each ground section to e-valuate the histological difference. Results A clear implant-bone interface, as well as the osteoblasts, nucleus and oste-oid had been distinctively demonstrated in the section stained by methylene blue-acid fuchsin. There was a relatively blurring delimitation between the new bone and the old bone. In the section stained by Goldner' s trichrome, the different stages of calcification were clearly shown, whereas the osteocytes were unclear and had an unclear boundary with the oste-oid. In the toluidine blue stained section the delimitation between the new bone and the lamellar bone was shown, whereas the implant-bone interface was blurring and the osteocytes were unclear too. Conclusion The Goldner' s trichrome and the toluidine blue are more suitable for showing the status of osteogenesis, and the former is better in showing the different stage of calcification. The Goldner's triehrome and methylene blue-acid fuchsin are better when displaying the implant-bone interface. The methylene blue-acid fuchsin is more suitable for the cytologieal analysis on ground section with implant.关键词
特殊染色法/不脱钙种植体骨磨片/组织形态学/成骨细胞Key words
Specific staining/ Undecalcified bone-implant section/ Histomorphology/ Osteoblasts分类
口腔医学引用本文复制引用
范芹,徐世同,刘建国,吕聪..3种特殊染色法显示种植体骨磨片组织学效果比较[J].广东牙病防治,2012,20(6):285-287,3.基金项目
广州市海珠区科技重点攻关项目(2007-Y-005) （2007-Y-005）
