广东医学2012，Vol.33Issue(15)：2233-2236,4.
原发性干燥综合征患者单核源性树突状细胞对聚肌苷酸胞苷酸刺激的反应性
Monocyte-derived dendritic cells from primary Sj(o)gren′s syndrome patients were hypersensitive to polyinosinic polycytidylic acid stimulation
摘要
Abstract
Objective To investigate the potential correlation between pathogenesis of primary Sjogren' s syndrome ( pSS ) and the function of monocyte-derived dendritic cells. Methods Monocytes from 11 pSS patients and 11 healthy controls were isolated and induced to dendritic cells in the presence of IL-4 and GM-CSF. Polyinosinic polycytidylic acid ( polyI: C, 50 μg/mL ) was used to simulate the monocyte - derived dendritic cells. Serial cytokines and TLR3 mRNA were assessed by ELISA and RT - PCR, respectively, at the 6th, 12th and 14th hour after polyI: C treatment. Results Significantly more IFN -α, IFN -β and TNF - α were produced in monocyte - derived dentritic cells from pSS patients when challenged with polyI: C. Although TLR3 mRNA expression was down - regulated by polyI: C stimulation, it was impaired in pSS patients. Conclusion The Hypersensitivity to polyI: C, which may associated with TLR3 abnormality , of the monocyte-derived dendritic cells from pSS patients was identified. The abnormality of monocyte-derived dendritic cells from pSS patients may involved in the pathogenesis of pSS.关键词
原发性干燥综合征/单核源性树突状细胞/天然免疫/聚肌苷酸胞苷酸/Toll样受体/细胞因子Key words
primary Sjogren syndrome, monocyte-derived dendritic cells, innate immune, polyinosinic polycytidylic acid, Toll-like receptors, cytokine引用本文复制引用
谷明莉,孙懿,秦琴,黄元兰,陈孙孝,邓安梅..原发性干燥综合征患者单核源性树突状细胞对聚肌苷酸胞苷酸刺激的反应性[J].广东医学,2012,33(15):2233-2236,4.基金项目
国家自然科学基金面上项目(编号:30972730),上海市科学技术委员会科研计划项目(编号:09JC1405400,11JC1410902) （编号:30972730）
